Polymerase chain reactionis a scientific technique

This provided an opportunity to overcome the difficulty with the need to repetitively adding deoxyribonucleic acid polymerase to the PCR response because it was being undo by heating. By using deoxyribonucleic acid polymerase from Thermus aquaticus (Taq), the heat fuss could be overcome, and the result was a speeding up of the change PCR process. Taq became a major tool for molecular biology because the PCR system could now be simplified and automated (Polymerase, 2004).

PCR is now carried appear in a thermocycler, a machine that heats and cools the reaction tubes in spite of appearance it to the precise temperatures required for each step in the PCR reaction (Polymerase, 2004). A heating lid is placed on slide by of the tubes, or a layer of oil is placed on top of the reaction mixture to prevent evaporation during the processing. The bit consists of about 20 to 30 cycles of three steps each. In the first step, the DNA is heated to 96oC to separate the strands. This step is cognize as the melting step, and breaks apart the hydrogen bonds connecting the both DNA strands. In the second step, the annealing step, the tempera

2. If the G-C content of Micrococcus is 66-75 moles %, and that of Staphylococcus is 30-40 moles %, one would conclude that the two may be somewhat related, just not closely. there is too much of a difference in the G-C percentages for them to be closely related genera.

Taxonomically, Staphylococcus is in the bacterial family Micrococcaceae, but the staphylococci are phylogenetically unrelated to any separate genera in the family (Staphylococcus, 2002). There are a large event of genetic criteria which indicate that the genus Staphylococcus forms a coherent and unclouded group which is widely divergent from the genus Micrococcus. The genus Staphylococcus belongs to the broad Bacillus-Lactobacillus-Streptococcus crew on the basis of 16s RNA analysis.

http://www.niaid.nih.gov/daids/vaccine/live.htm

Staphylococcus. (2002). Retrieved at:

Finding cures. Saving children. (2003). Retrieved at:

ture is lowered to allow primers to bond themselves to single strands of DNA. Primers are short, artificial DNA strands, which anneal to the DNA template at the starting and ending points where the DNA polyme
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